NGS Library Pooling

Pooling Next Generation Sequencing (NGS) libraries is an essential step in the overall NGS sequencing workflow. After adapter ligation, samples from a PCR plate are pooled and sequenced at the same time. Library pooling saves time and money during NGS by enabling multiple samples to be simultaneously sequenced and aligned in silico.

Benefits of NGS library pooling with VBLOK200 instead of pipetting include:

  • Reduced pipette tip waste and lowered cost of pooling NGS libraries.
  • Increased recovery of valuable, low abundance samples from single cell sequencing workflows.
  • Recovery of the maximum available volume of NGS libraries.

How do I pool NGS Libraries?

NGS libraries are traditionally pooled via pipette-based aspirating from a PCR plate and dispensing into a receptacle. Liquid retention on pipette tips and multi-well plates makes this a loss-prone procedure. For the precious and low-abundance samples generated by RNASeq and single cell sequencing workflows, maximizing library yield is key to generating high quality data.

Using the ClickBio VBLOK to improve your workflow

The ClickBio VBLOK200’s patented centrifugal collection technology enables increased volume recovery from multi-well plates while simultaneously saving pipette tips. By reducing waste and increasing library recovery, The VBLOK200 enables a more efficient low waste workflow and higher quality data generation.

In addition to higher library recovery from the multi-well plates, VBLOK200’s geometry enables full recovery of the prepared library from the collection plate.  



The Easy Choice for NGS Library Prep

ClickBio’s VBLOK200 is the easiest choice you’ll make all day. Designed to save pipette tips and time when pooling NGS libraries, simply invert a plate in the VBLOK and centrifuge to collect your prepared libraries.